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Trypsin Synthesis and Storage as Zymogen in the Midgut Gland of the Shrimp Litopenaeus Vannamei

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Abstract An immunological approach was used to elucidate whether trypsin is synthesized and stored as trypsinogen in the midgut gland of the shrimp Litopenaeus vannamei. Two peptides were constructed using sequences deduced from known shrimp genes: trypsinogen activation peptide and an internal sequence. These peptides were used as haptens to elicit antibodies in rabbits. Specific antibodies were used to detect trypsinogen by Western blot and in histological sections of the midgut gland. Trypsinogen was found by Western blot and was localized into the midgut gland B cells by using immunohistology. In fed shrimp, trypsinogen associated with food particles was found in the lumen of the midgut gland tubules as well. Our results show that regulation of shrimp trypsin activity is similar to that of frequent feeder species, in which trypsin is stored as a zymogen, waiting for secretion and activation.

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1. Babu D. E. , Manjulatha C. 1995 "Zymogen secreting cells in the hepatopancreatic duct of Pagurus bernhardus (Linnaeus, 1758) and Clibanarius longitarsus (de Haan, 1849) (Anomura)." Crustaceana Vol 68 616 628
2. Bell T. A. , Lightner D. V. 1988. A Handbook of Normal Penaeid Shrimp Histology. World Aquaculture Society. University of Arizona, Tussan, Arizona. 112 pp
3. Ceccaldi H. J. 1989 "Anatomy and physiology of digestive tract of crustacean decapods reared in aquaculture." Advances in Tropical Aquaculture Vol 9 243 259
4. Chan S. M. , Ranking S. M. , Keeley L. L. 1988 "Characterization of the molt stages in Penaeus vannamei: setogenesis and hemolymph levels of total protein, ecdysteroids, and glucose." Biological Bulletin (Woods Hole) Vol 175 185 192
5. Corring T. , Juste C. , Lhoste E. F. 1989 "Nutritional regulation of pancreatic and biliary secretions." Nutrition Research Reviews Vol 2 161 180
6. Dall W. 1992. Feeding, digestion and assimilation in Penaeidae.Proceedings of the Aquaculture Nutrition Workshop: 5762
7. Dyrberg T. , Oldstone M. B. 1986 "Peptides as antigens. Importance of orientation." Journal of Experimental Medicine Vol 164 1344 1349
8. Fukuoka S. , Kawajiri H. , Fushiki T. , Takahashi K. , Iwai K. 1986 "Localization of pancreatic enzyme secretion-stimulating activity and trypsin inhibitory activity in zymogen granule of the rat pancreas." Biochimica et Biophysica Acta Vol 884 18 24
9. Garca-Carreño F. L. , Haard N. 1993 "Characterization of proteinase classes in langostilla (Pleuroncodes planipes) and crayfish (Pacifastacus astacus) extracts." Journal of Food Biochemistry Vol 17 97 113
10. Garca-Carreño F. L. , Hernandez-Cortes M. P. , Haard N. 1994 "Enzymes with peptidase and proteinase activity from the digestive system of a freshwater and marine decapod." Journal of Agricultural and Food Chemistry Vol 42 1456 1461
11. Gates B. J. , Travis J. 1969 "Isolation and comparative properties of shrimp trypsin." Biochemistry Vol 9 4483 4489
12. Gibson R. , Barker P. 1979 "The decapod hepatopancreas." Oceanography and Marine Biology Vol 17 285 346
13. Hara H. , Hashimoto N. , Akatsuka N. , Kasai T. 1999 "Induction of pancreatic trypsin by dietary amino acids in rats: four trypsinogen isozymes and cholecystokinin messenger RNA." Journal of Nutrition Biochemistry Vol 11 52 59
14. Harlow E. , Lane D. 1988. Antibodies, a Laboratory Manual. Cold Spring Harbor Laboratory, U.S.A. 726 pp
15. Heijne Gvon 1987. Sequence Analysis in Molecular Biology. Treasure Trove or Trivial Pursuit. Academic Press, San Diego. 188 pp
16. Hernndez-Cortes P. , Cerenius L. , Garcia-Carreño F. , Sderhll K. 1999a "Trypsin from Pacifastacus leniusculus hepatopancreas: purification and cDNA cloning of the synthesized zymogen." Biological Chemistry Vol 380 499 501
17. Hernndez-Cortes P. , Quadros-Seiffert W. , Navarrete del Toro M. A. , Portillo G. , Colado G. , Garca-Carreño F. L. 1999b "Rate of ingestion and proteolytic activity in digestive system of juvenile white shrimp, Penaeus vannamei, during continual feeding." Journal of Applied Aquaculture Vol 9 35 45
18. Hernndez-Cortes P. , Whitaker J. R. , Garcia-Carreño F. L. 1997 "Purification and characterization of chymotrypsin from Penaeus vannamei (Crustacea:Decapoda)." Journal of Food Biochemistry Vol 21 497 514
19. Hogue W. V. 1997 "Cn3d: a new generation of three-dimensional molecular structure viewer." Trends in Biochemical Sciences Vol 22 314 316
20. Klein B. , Le Moullac G. , Sellos D. , Van Wormhoudt A. 1996 "Molecular cloning and sequencing of trypsin cDNAs from Penaeus vannamei (Crustacea, Decapoda): use in assessing gene expression during the molt cycle." International Journal of Biochemistry and Cell Biology Vol 28 551 563
21. Laemmli U. K. 1970 "Cleavage of structural proteins during the assembly of the head of bacteriophage T4." Nature Vol 227 680 685
22. Lehnert S. A. , Johnson S. E. 2002 "Expression of hemocyanin and digestive enzyme messenger RNAs in the hepatopancreas of the black tiger shrimp Penaeus monodon." Comparative Biochemistry and Physiology Part B Vol 133 163 171
23. Lemos D. , Ezquerra J. M. , Garca-Carreño F. L. 2000 "Protein digestion in penaeid shrimp: digestive proteinases, proteinase inhibitors and feed digestibility." Aquaculture Vol 186 89 105
24. Le Moullac G. L. , Klein B. , Sellos D. , Van Wormhoudt A. 1996 "Adaptation of trypsin, chymotrypsin and -amylase to casein level and protein source in Penaeus vannamei (Crustacea Decapoda)." Journal of Experimental Marine Biology and Ecology Vol 208 107 125
25. Light A. , Janska H. 1989 "Enterokinase (enteropeptidase): comparative aspects." Trends in Biochemical Sciences Vol 14 110 112
26. Lu P. J. , Liu H. C. , Tsai I. H. 1990 "The midgut trypsins of shrimp (Penaeus monodon). High effieciency toward native protein substrates including collagens." Biological Chemistry Vol 371 851 859
27. Martin D. W. , Rodwell V. W. , Mayer P. A. 1982. Bioqumica de Harper, El manual moderno S.A de C.V. Mex. Mex. 633 pp
28. Muller H. M. , Crampton J. M. , della Torre A. , Sinden R. , Crisanti A. 1993 "Members of a trypsin gene family in Anopheles gambiae are induced in the gut by blood meal." European Molecular Biology Organization Journal Vol 12 2891 2900
29. Noriega F. G. , Wells M. A. 1999 "A molecular view of trypsin synthesis in the midgut of Aedes aegypti." Journal of Insect Physiology Vol 45 613 620
30. Prez Farfante I. , Kensley B. 1997. Penaeoid and Sergestoid Shrimps and Prawns of the World. Keys and Diagnoses for the Families and Genera. Memoires du Museum National d'Historie Naturelle, Paris, France. 233 pp
31. Rypniewski W. R. , Perrakis A. , Vorgias C. E. , Wilson K. S. 1994 "Evolutionary divergence and conservation of trypsin." Protein Engineering Vol 7 57 64
32. Secor S. M. 2001 "Regulation of digestive performance: a proposed adaptive response." Comparative Biochemistry and Physiology Part A Vol 128 565 577
33. Secor S. M. , Diamond J. M. 2000 "Evolution of regulatory responses to feeding in snakes." Physiological and Biochemical Zoology Vol 73 123 141
34. Tidwell J. H. , Allan G. L. 2001 "Fish as food: aquaculture's contribution. Ecological and economic impacts and contributions of fish farming and capture fisheries." European Molecular Biology Organization Reports Vol 2 958 963
35. Towbin H. , Staehelin T. , Gordon J. 1979 "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications." Proceedings of the National Academy of Sciences of the United States of America Vol 76 4350 4354
36. Tsu C. A. , Craik C. S. 1996 "Substrate recognition by recombinant serine collagenase from Uca pugilator." Journal of Biological Chemistry Vol 271 11563 11570
37. Wormhoudt A. Van , Sellos D. , Donval A. , Plaire-Goux S. , Le Moullac G. 1995 "Chymotrypsin gene expression during the intermolt cycle in the shrimp Penaeus vannamei (Crustacea Decapoda)." Experientia Vol 51 159 163
38. Zwilling R. , Doersam H. , Torff H. , Rold J. 1981 "Low molecular mass protease: evidence for a new family of proteolytic enzymes." Federation of European Biochemical Societies Letters Vol 127 75 78
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/content/journals/10.1651/c-2423
2004-01-01
2016-12-08

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