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Circulating Ecdysteroid Concentrations in Alaskan Dungeness Crab (Cancer Magister)

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AbstractThe currently accepted reproductive cycle of Alaskan female Dungeness crab (Cancer magister) is that females molt and mate during summer and fall, extrude eggs in the fall, and incubate eggs through the winter until larval hatching in May and June. In this cycle, molting and mating are coincident with ovarian maturation. In this study, we quantified circulating concentrations of hemolymph ecdysteroids (molting hormones) in laboratory and field sampled crabs using an enzyme-linked immunosorbent assay (ELISA), for molt status assessment of individuals. Captive female crabs from southeastern Alaska (mean CW = 134.8 mm; n = 48) had a typical crustacean molt cycle profile of circulating ecdysteroids. Concentrations of ecdysteroids were low during intermolt (20.3 ± 0.7 ng/ml), maximal during premolt (1886.5 ± 186.2 ng/ml) 15 days before ecdysis and precipitously declined to low levels (< 90 ng/ml) 5 days prior to ecdysis. The duration of premolt was 150 days. Crabs held in captivity either molted (67%), extruded eggs (7%), or demonstrated no reproductive or molting activity (26%). Peak molting occurred in November for crabs held in the laboratory. Most (98%) of the female crabs sampled (n = 579) in Port Frederick, Alaska during the expected premolt period (May-July), had intermolt ecdysteroid concentrations. The capability to predict ecdysis 150 days prior to ecdysis by measuring ecdysteroid concentrations provides a useful tool to assess the molt status and timing of ecdysis in Alaskan Dungeness crab populations.

Affiliations: 1: a(JDT, SA) University of Alaska Fairbanks, P.O. Box 1329, Seward, Alaska 99664, U.S.A., Alaska SeaLife Center, 301 Railway Avenue, Seward, Alaska 99664, U.S.A. ( jamie_thomton@alaskasealife.org; shannon_atkinson@alaskasealife.org) ; 2: b(SLT, correspondence) University of Alaska Southeast, 11120 Glacier Hwy, Juneau, Alaska 99801, U.S.A. ( sherry.tamone@uas.alaska.edu)

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1. Borst D. W., Laufer H., Landau M., Chang E. S., Hertz W. A., Baker F. C., Schooley D. A. 1987 "Methyl farnesoate and its role in crustacean reproduction and development." Insect Biochemistry Vol 17 1123 1127
2. Borst D. W., Martin M., Chang E. S. 1988 "Regulation of methyl farnesoate levels in hemolymph of Homarus americanus." American Zoologist Vol 28 82A
3. Butler T. H. 1960 "Maturity and breeding of the Pacific edible crab, (Cancer magister Dana)." Journal of the Fisheries Research Board of Canada Vol 17 641 646
4. Byard E. H., Aiken D. E. 1984 "The relationship between molting, reproduction, and a hemolymph female-specific protein in the lobster, Homarus americanus." Comparative Biochemistry and Physiology Vol 77A 749 757
5. Chang E. S. 1985 "Hormonal control of molting in decapod crustacea." American Zoologist Vol 25 179 185
6. Chang E. S., Bruce M. J. 1980 "Ecdysteroid titers of juvenile lobsters following molt induction." Journal of Experimental Zoology Vol 214 157 160
7. Chang E. S., O'Connor J. D. 1977 "Secretion of α-ecdysone by crab Y-organs in vitro." Proceedings of the National Academy of Sciences of the USA Vol 74 615 618
8. Gunamalai V., Kirubagaran R., Subramonium T. 2004 "Hormonal coordination of molting and female reproduction by ecdysteroids in the mole crab Emerita asiatica (Milne Edwards)." General and Comparative Endocrinology Vol 138 128 138
9. Hampshire R., Horn D. H S. 1966 "Structure of crustecdysone, a crustacean moulting hormone." Chemical Communications Vol 1966 37 38
10. Hankin D. G., Diamond N., Mohr M. S., Ianelli J. 1989 "Growth and reproductive dynamics of adult female Dungeness crabs (Cancer magister) in northern California." Journal du Conseil International Pour l'Exploration de la Mer Vol 46 94 108
11. Hankin D. G., Xue Q. 1996 "Preliminary report on interannual variation in size-specific molting probabilities of adult female Dungeness Crabs (Cancer magister) in northern California. Proceedings of the International Symposium on Biology, Management, and Economics of Crabs from High Latitude Habitats." University of Alaska Sea Grant College Program Report No. 96-02: 425-441
12. Hopkins P. M. 1983 "Patterns of serum ecdysteroids during induced and uninduced proecdysis in the fiddler crab, Uca pugilator." General and Comparative Endocrinology Vol 52 350 356
13. Jensen G. C., Armstrong D. A. 1987 "Range extensions of some northeastern Pacific Decapoda." Crustaceana Vol 52 215 217
14. Kingan T. G. 1989 "A competitive enzyme-linked immunosorbent assay: applications in the assay of peptides, steroids, and cyclic nucleotides." Analytical Biochemistry Vol 183 283 289
15. Koeneman T. M. 1985 "A brief review of the commercial fisheries for Cancer magister in southeast Alaska and Yakutat waters, with emphasis on recent seasons. Proceedings of the Symposium on Dungeness Crab Biology and Management." Alaska Sea Grant Report No. 85-3: 61-76
16. Lehman C., Osborn O. 1970 "Dungeness crab research." Completion report, Alaska Department of Fish and Game, Juneau, Alaska. 43 pp
17. Lyle W. G., MacDonald C. D. 1983 "Molt stage determination in the Hawaiian spiny lobster Panulirus marginatus." Journal of Crustacean Biology Vol 3 208 216
18. MacKay D. C. 1943 "The behavior of the Pacific edible crab Cancer magister Dana." Journal of Comparative Physiology Vol 36 255 268
19. Mattson M. P., Spanziani E. 1987 "Demonstration of protein kinase C activity in crustacean Y-organs, and partial definition of its role in regulation of ecdysteroidogenesis." Molecular and Cellular Endocrinology Vol 49 159 171
20. Miller T. W., Hankin D. G. 2002 "Setal stage duration of female adult Dungeness crab (Cancer magister)." Crabs in Cold Water Regions Vol AK-SG-02-01 9 15
21. Naya Y., Ohnishi M., Ikeda M., Miki W., Nakanishi K. 1989 "What is molt-inhibiting hormone? The role of an ecdysteroidogenesis inhibitor in the crustacean molting cycle." Proceedings of the National Academy of Sciences, USA Vol 86 6826 6829
22. Okazaki R. K., Chang E. S. 1991 "Ecdysteroids in the embryos and sera of the crabs, Cancer magister and Cancer anthonyi." General Comparative Endocrinology Vol 81 174 186
23. Shirley S. M., Shirley T. C. 1988 "Appendage injury in Dungeness crabs, Cancer magister, in southeastern Alaska." Fishery Bulletin Vol 86 156 160
24. Snow C. D., Nielson J. R. 1966 "Premating and mating behavior of the Dungeness crab (Cancer magister Dana)." Journal of the Fisheries Research Board of Canada Vol 23 1319 1323
25. Somerton D. A., Macintosh R. A. 1983 "Weight-size relationships for three populations in Alaska of the blue king crab, Paralithodes platypus (Brandt, 1850) (Decapoda, Lithodidae)." Crustaceana Vol 45 169 175
26. Soumoff C., O'Connor J. D. 1982 "Repression of Y-organ secretory activity by molt inhibiting hormone in the crab Pachygrapsus crassipes." General Comparative Endocrinology Vol 48 432 439
27. Soumoff C., Skinner D. M. 1983 "Ecdysteroid titers during the molt cycle of the blue crab resemble those of other crustacea." Biological Bulletin Vol 165 321 329
28. Stone R. P., O'Clair C. E. 2001 "Seasonal movements and distribution of Dungeness crabs Cancer magister in a glacial southeastern Alaska estuary." Marine Ecology Progress Series Vol 214 167 176
29. Subramoniam T. 2000 "Crustacean ecdysteroids in reproduction and embyrogenesis." Comparative Biochemistry and Physiology part C Vol 125 135 156
30. Sudha K., Anilkumar G. 1996 "Seasonal growth and reproduction in a highly fecund brachyuran crab, Metapograpsus messor (Forskal, Grapsidae)." Hydrobiologia Vol 319 15 21
31. Swiney K. M., Shirley T. C. 2001 "Gonad development of southeastern Alaskan Dungeness Crab, Cancer magister, under laboratory conditions." Journal of Crustacean Biology Vol 21 897 904
32. Swiney K. M., Shirley T. C., Taggart S. J., O'Clair C. E. 2003 "Dungeness crab, Cancer magister, do not extrude eggs annually in southeastern Alaska: an in situ study." Journal of Crustacean Biology Vol 23 30 38
33. Tamone S. L., Adams M. M., Dutton J. M. 2005 "Effect of eyestalk ablation on circulating ecdysteroids in hemolymph of snow crabs, Chionoecetes opilio: physiological evidence for a terminal molt." Integrative and Comparative Biology Vol 45 166 171
34. Tamone S. L., Chang E. S. 1993 "Methyl farnesoate stimulates ecdysteroid secretion from crab Y-organs in vitro." General and Comparative Endocrinology Vol 89 425 432
35. Wainwright T. C., Armstrong D. A. 1993 "Growth patterns in the Dungeness crab (Cancer magister Dana): synthesis of data and comparison of models." Journal of Crustacean Biology Vol 13 36 50
36. Webster S. G. 1993 "High-affinity binding of putative moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) to membrane-bound receptors on the Y-organ of the shore crab Carcinus maenas." Proceedings of the Royal Society of London Series B Biological Sciences Vol 251 53 59
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/content/journals/10.1651/c-2625.1
2006-01-01
2016-12-09

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