Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Immunolocalization of Na+,K+-ATPase in Osmoregulatory Organs During the Embryonic and Post-Embryonic Development of the Lobster Homarus Gammarus

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Journal of Crustacean Biology

Abstract Immunolocalization and ontogenetical changes in Na+,K+-ATPase were investigated in the antennal glands, intestine, and branchial cavity in developing Homarus gammarus. The antennal glands lined by undifferentiated cells are detectable in embryos at stage EI 150 μm (EI: eye index; 640 μm at hatching). From a mesodermic sac and an ectodermic tubular epithelium (EI 225 μm), they develop into coelomosac, labyrinth, and bladder up to larval stage II. In larval stage III, the end parts of the labyrinth fold inward. In the first post-larvae, the coelomosac is completely enclosed in the folded labyrinth, there is no nephridial tubule. The definitive organization of the antennal gland found in adults is achieved in post-larval stage V. At EI 425 μm, the intestine epithelium is composed of cuboidal cells, and the branchial cavity is already formed at EI 325 μm. The presence of Na+,K+-ATPase was detected on the basolateral side of ionocytes lining the epithelium sac of the antennal gland starting at EI 425 μm, in the intestine at EI 625 μm, and in the epipodites of the branchial cavity at EI 525 μm. In post-larvae (stage V), a strong fluorescence was also found on the inner-side of the branchiostegite epithelium. Thus the ontogeny of the osmoregulatory epithelia is completed only after metamorphosis and it conditions the occurrence of the adult pattern of osmoregulation.

Affiliations: 1: a (SK; GC; MCD; correspondence) Equipe Adaptation Ecophysiologique et Ontogenèse, UMR 5171 GPIA, Université Montpellier II, cc 092, 34095 Montpellier cedex 05, France. (;


Full text loading...


Data & Media loading...

1. Anger K. 2001 "The Biology of Decapoda Crustacean Larvae, Crustacean Issues 14." A. A. Balkema, Lisse. 419 p
2. Barradas C. , Wilson J. M. , Dunel-Erb S. 1999 "Na,K-ATPase activity and immunocytochemical labelling in podobranchial filament and lamina of the freshwater crayfish Astacus leptodactylus Eschscholtz: evidence for the existence of sodium transport in the filaments." Tissue and Cell Vol 31 523 528
3. Bouaricha N. , Charmantier G. , Charmantier-Daures M. , Thuet P. , Trilles J-P. 1991a "Ontognse de l'osmoregulation chez la crevette Penaeus japonicus." Cahiers de Biologie Marine Vol 322 149 158
4. Bouaricha N. , Charmantier-Daures M. , Thuet P. , Trilles J-P. , Charmantier G. 1991b "Ontogeny of osmoregulatory structures in the shrimp Penaeus japonicus (Crustacea, Decapoda)." Biological Bulletin Vol 186 29 40
5. Campbell P. J. 1986 "Growth of tagged American lobster Homarus americanus in the Bay of Fundy." Canadian Journal of Fisheries and Aquatic Science Vol 40 1667 1675
6. Campbell P. J. , Jones M. B. 1990 "Water permeability of Palaemon longirostris and other euryhaline caridean prawns." Journal of Experimental Biology Vol 150 145 158
7. Charmantier G. 1998 "Ontogeny of osmoregulation in crustaceans: a review." Invertebrate Reproduction and Development Vol 33 177 190
8. Charmantier G. , Aiken D. E. 1987 "Osmotic regulation in late embryos and prelarvae of the American lobster Homarus americanus (Crustacea, Decapoda)." Journal of Experimental Marine Biology and Ecology Vol 109 101 108
9. Charmantier G. , Charmantier-Daures M. , Bouaricha N. , Thuet P. , Aiken D. E. , Trilles J-P. 1988 "Ontogeny of osmoregulation and salinity tolerance in two decapod crustaceans: Homarus americanus and Penaeus japonicus." Biological Bulletin Vol 175 102 110
10. Charmantier G. , Mounet-Guillaume R. 1992 "Temperature specific rate of embryonic development of the European lobster Homarus gammarus (L)." Journal of Experimental Marine Biology and Ecology Vol 160 61 66
11. Charmantier G. , Thuet P. , Charmantier-Daures M. 1984 "La regulation osmotique et ionique chez Homarus gammarus (L.) (Crustacea: Decapoda)." Journal of Experimental Marine Biology and Ecology Vol 76 191 199
12. Charmantier G. , Haond C. , Lignot J. H. , Charmantier-Daures M. 2001 "Ecophysiological adaptation to salinity throughout a life cycle: a review in homarid lobsters." Journal of Experimental Biology Vol 204 967 977
13. Cieluch U. , Anger K. , Aujoulat F. , Buchholz F. , Charmantier-Daures M. , Charmantier G. 2004 "Ontogeny of osmoregulatory structures and functions in the green crab, Carcinus maenas (Crustacea, Decapoda)." Journal of Experimental Biology Vol 207 325 335
14. Cieluch U. , Charmantier G. , Grousset E. , Charmantier-Daures M. , Anger K. 2005 "Osmoregulation, immunolocalization of Na/K-ATPase, and ultrastructure of branchial epithelia in the developing brown shrimp, Crangon crangon (Decapoda, Caridea)." Physiological and Biochemical Zoology Vol 78 1017 1025
15. Dall W. 1970 "Osmoregulation in the lobster Homarus americanus." Journal of Fisheries Research Board Canada Vol 27 1123 1130
16. De Renzis G. , Bornancin M. 1984 "Ion transport and gill ATPase." pp. 65-104. In, Hoar, W. S., D. J. Randall (eds.), Fish physiology, gills. Vol. XB. Academic Press, Orlando
17. Farmer L. 1980 "Evidence for hyporegulation in the calanoid copepod Acartia tonsa." Comparative Biochemistry and Physiology Vol 65A 359 362
18. Felder J. , Felder D. , Hand S. 1986 "Ontogeny of the osmoregulation in the estuarine ghost shrimp Callianassa jamaicense var. louisianensis Schmidtt (Decapoda, Thalassinidae)." Journal of Experimental Marine Biology and Ecology Vol 99 91 105
19. Freire C. A. , Mc Namara J. C. 1995 "Fine structure of the gills of the fresh-water shrimp Macrobrachium olfersii (Decapoda): effect of acclimation to high salinity medium and evidence for involvement of the lamellar septum in ion uptake." Journal of Crustacean Biology Vol 15 103 116
20. Fuller E. G. , Highison G. J. , Brown F. , Bayer C. 1989 "Ultrastructure of the crayfish antennal gland revealed by scanning and transmission electron microscopy combined with ultrasonic microdissection." Journal of Morphology Vol 200 9 15
21. Green J. W. , Harsch H. , Barr L. , Prosser C. L. 1959 "The regulation of water and salt by the fiddler crab Uca pugnax and Uca pugilator." Biological Bulletin Vol 116 76 87
22. Goodrich E. S. 1946 "Nephridia and genital ducts since 1895." Quarterly Journal of Microscopic Science Vol 68 113 393
23. Haond C. , Flik G. , Charmantier G. 1998 "Confocal laser scanning and electron microscopical studies on osmoregulatory epithelia in the branchial cavity of the lobster Homarus gammarus." Journal of Experimental Biology Vol 201 1817 1833
24. Heeg J. , Cannone J. 1966 "Osmoregulation by means of a hitherto unsuspected osmoregulatory organ in two grapsid crabs." Zoologica Africana Vol 2 127 129
25. Holdich D. M. , Ratcliffe N. 1970 "A light and electron microscopic study of the hindgut of the herbivorous isopod Dynamene bidentata (Crustacea, Pericarida)." Zeitschrift fur Zellforschung und mikroskopische Anatomie Vol 11 209 227
26. Holdich D. M. , Mayes K. R. 1975 "A fine structural re-examination of the so-called midgut of the isopod Porcellio." Crustaceana Vol 29 186 192
27. Holliday C. W. 1980 "Magnesium transport by the urinary bladder of the crab, Cancer magister (Dana)." Journal of Experimental Biology Vol 85 187 201
28. Holliday C. W. , Miller D. S. 1984 "Cellular mechanisms of organic anion transport in crustacean renal tissue." American Zoologist Vol 24 275 284
29. Holliday C. W. , Mykles D. L. , Terwilliger R. C. , Dangott L. J. 1980 "Fluid secretion by the midgut caeca of the crab Cancer magister." Journal of Experimental Biology Vol 85 187 201
30. Hubschman J. H. 1971 "Transient larval glands in Palamonetes." In, D. J. Crisp, ed. Fourth European Mar. Biol. Symposium. Cambridge University Press, New York
31. Hughes J. T. , Shleser R. A. , Tchobanoglous G. 1974 "A rearing tank for lobster and other aquatic species." The Progressive Fish-Culturist Vol 36 129 133
32. Johnson P. T. 1980 "Histology of the Blue Crab Callinectes sapidus." Praeger, New York
33. Jury S. H. , Kinnison M. T. , Howell W. H. , Watson W. H. III 1994 "The behaviour of lobsters in response to reduced salinity." Journal of Experimental Marine Biology and Ecology Vol 180 23 37
34. Khodabandeh S. , Kutnik M. , Charmantier-Daures M. , Charmantier G. 2002 "Ontogeny of the antennal glands in the Crayfish Astacus leptodactylus." Integrative and Comparative Biology Vol 42 1255
35. Khodabandeh S. , Charmantier G. , Blasco C. , Grousset E. , Charmantier-Daures M. 2005a "Ontogeny of the antennal glands in the Crayfish, Astacus leptodactylus (Crustacea, Decapoda): anatomical and cell differentiation." Cell & Tissue Research Vol 319 153 165
36. Khodabandeh S. , Kutnik M. , Aujoulat F. , Charmantier G. , Charmantier-Daures M. 2005b "Ontogeny of the antennal glands in the Crayfish, Astacus leptodactylus (Crustacea, Decapoda): immunolocalisation of Na,K-ATPase." Cell & Tissue Research Vol 319 167 174
37. Khodabandeh S. , Charmantier G. , Charmantier-Daures M. 2005c "Ultrastructural studies and Na,K-ATPase immunolocalization in the antennal urinary glands of the lobster Homarus gammarus (Crustacea, Decapoda)." Journal of Histochemistry & Cytochemistry Vol 53 1203 1214
38. Lawton P. , Lavalli K. L. 1995 "Postlarval, juvenile, adolescent, and adult ecology." In, J. R. Factor (ed.), Biology of the lobster Homarus americanus. Academic Press. New York
39. Lignot J. H. , Charmantier-Daures M. , Charmantier G. 1999 "Immunolocalization of Na,K-ATPase in the organs of the branchial cavity of the European lobster Homarus gammarus (Crustacea, Decapoda)." Cell and Tissue Research Vol 296 417 426
40. Lignot J. H. , Charmantier G. 2001 "Immunolocalization of Na,K-ATPase in the branchial cavity during the early development of the European lobster Homarus gammarus (Crustacea, Decapoda)." Journal of Histochemistry and Cytochemistry Vol 49 1013 1023
41. Lignot J. H. , Susanto G. N. , Charmantier-Daures M. , Charmantier G. 2005 "Immunolocalization of Na,K-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)." Cell and Tissue Research Vol 319 331 339
42. Lucu C. , Towle D. W. 2003 "Na,K-ATPase in the gills of aquatic crustacea." Journal of Comparative Biochemistry and Physiology Vol 135 A 195 214
43. Mantel L. H. 1968 "The foregut of Gecarcinus lateralis as an organ of salt and water balance." American Zoologist Vol 8 433 442
44. Mantel L. H. , Farmer L. L. 1983 "Osmotic and ionic regulation." pp. 53-161. In, D. E. Bliss (ed.), The Biology of Crustacea. Vol. 5. Internal Anatomy and Physiological Regulation. Academic Press, London
45. Mykles D. L. 1977 "The ultrastructure of the posterior midgut caecum of Pachygrapsus crassipes (Decapoda, Brachyura) adapted to low salinity." Tissue and Cell Vol 9 681 691
46. Mykles D. L. 1979 "Ultrastructure of alimentary epithelia of lobsters, Homarus americanus and H. gammarus and crab, Cancer magister." Zoomorphology Vol 92 201 215
47. Palackal T. , Faso L. , Zung J. L. , Vernon G. , Witkus R. 1984 "The ultrastructure of the hindgut epithelium of terrestrial isopods and it role in osmoregulation." Symposia of the Zoological Society of London Vol 53 185 198
48. Pqueux A. 1995 "Osmotic regulation in crustaceans." Journal of Crustacean Biology Vol 15 1 60
49. Perkins H. C. 1972 "Developmental rates at various temperatures of embryos of the northern lobster (Homarus americanus Milne-Edwards)." Fisheries Bulletin Vol 70 95 99
50. Sarver R. G. , Flynn M. A. , Holliday C. W. 1994 "Renal Na, K-ATPase and osmoregulation in the crayfish, Procambarus clarkii." Comparative Biochemistry and Physiology Vol 107A 349 356
51. Schmidt-Nielsen B. , Davis L. E. 1968 "Transport and tubular intercellular spaces in reptilian kidneys." Science Vol 159 1105 1108
52. Thuet P. , Charmantier-Daures M. , Charmantier G. 1988 "Relation entre osmorgulation et activits d'ATPase Na,K et d'anhydrase chez larves et postlarves de Homarus gammarus (L.) (Crustacea: Decapoda)." Journal of Experimental Marine Biology and Ecology Vol 115 249 261
53. Towle D. W. 1981 "Role of Na,K-ATPase in ionic regulation by marine and estuarine animals." Marine Biology Letter Vol 2 107 121
54. Towle D. W. 1984a "Membrane-bound ATPase in arthropod ion-transporting tissues." American Zoologist Vol 24 77 185
55. Towle D. W. 1984b "Regulatory functions of Na,K-ATPase in marine and estuarine animals." pp. 157-170. In, A. Pqueux, L. Bolis (eds.), Heidelberg. Springer Verlag, New York. Tokyo
56. Towle D. W. , Weihrauch D. 2001 "Osmoregulation by gills of euryhaline crabs: molecular analysis of transporters." American Zoologist Vol 41 770 788
57. Trask T. 1974 "Laboratory-reared larvae of Cancer anthonyi (Decapoda: Brachyura) with a brief description of the internal anatomy of the megalopa." Marine Biology Vol 27 63 74
58. Ziegler A. 1997 "Immunocytochemical localization of Na,K-ATPase in the calcium transporting sternal epithelium of the terrestrial isopod Porcellio scaber L (Crustacea)." Journal of Histochemistry and Cytochemistry Vol 45 437 446

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Journal of Crustacean Biology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation